how to use graphpad prism for real time pcr

The new real-time PCR assay allows for accurate and rapid screening of C. The real-time PCR yielded results within 4 h of sample processing, compared to 4 to 14 days for culture, reducing turnaround time significantly.

auris DNA from 1% and 12% of swab and sponge samples with culture-negative results, indicating the presence of dead or culture-impaired C. Real-time PCR yielded positive results from 49 swab and 58 sponge samples, with 89% and 100% clinical sensitivity with regard to their respective culture-positive results. auris real-time PCR assay was evaluated by using 623 surveillance samples, including 365 patient swabs and 258 environmental sponges. The assay was highly specific, reproducible, and sensitive, with the detection limit of 1 C. To achieve these goals, we developed and validated a TaqMan-based real-time PCR assay targeting the internal transcribed spacer 2 ( ITS2) region of the ribosomal gene. auris is of primary importance for the implementation of public health measures to control the spread of infection. Candida auris is an emerging multidrug-resistant yeast causing invasive health care-associated infection with high mortality worldwide.

How to use graphpad prism for real time pcr